Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles.
نویسندگان
چکیده
Accurate concentration determination of subpopulations of extracellular vesicles (EVs), such as exosomes, is of importance both in the context of understanding their fundamental biological role and of potentially using them as disease biomarkers. In principle, this can be achieved by measuring the rate of diffusion-limited mass uptake to a sensor surface modified with a receptor designed to only bind the subpopulation of interest. However, a significant error is introduced if the targeted EV subpopulation has a size, and thus hydrodynamic diffusion coefficient, that differs from the mean size and diffusion coefficient of the whole EV population and/or if the EVs become deformed upon binding to the surface. We here demonstrate a new approach to determine the mean size (or effective film thickness) of bound nanoparticles, in general, and EV subpopulation carrying a marker of interest, in particular. The method is based on operating surface plasmon resonance simultaneously at two wavelengths with different sensing depths and using the ratio of the corresponding responses to extract the particle size on the surface. By estimating in this way the degree of deformation of adsorbed EVs, we markedly improved their bulk concentration determination and showed that EVs carrying the exosomal marker CD63 correspond to not more than around 10% of the EV sample.
منابع مشابه
Tunable Plasmonic Nanoparticles Based on Prolate Spheroids
Metallic nanoparticles can exhibit very large optical extinction in the visible spectrum due to localized surface plasmon resonance. Spherical plasmonic nanoparticles have been the subject of numerous studies in recent years due to the fact that the scattering response of spheres can be analytically evaluated using Mie theory. However a major disadvantage of metallic spherical nanoparticles is ...
متن کاملInvestigating the Effects of Some Capping Agents upon Gold Nanocrystals Stability, Size and Surface Plasmon Resonance
متن کامل
Hydrogen sensing by localized surface plasmon resonance in colloidal solutions of Au-WO3-Pd
Nowadays, hydrogen has attracted significant attention as a next generation clean energy source. Hydrogen is highly flammable, so detection of hydrogen gas is required. Gold nanoparticle based localized surface plasmon resonance (LSPR) is an advanced and powerful sensing technique, which is well known for its high sensitivity to surrounding refractive index change in the local environment. We p...
متن کاملAnalyzing the Optical Properties and Peak Behavior Due to Plasmon Resonance of Silver Cubic-Shape Nanostructures by Means of Discrete Dipole Approximation
In this article, the optical properties of silver cubic-shape nanostructures (SCNs) were analyzed by employing the discrete dipole approximation (DDA) in aqueous media. The absorption, dispersion and extinction cross-sections of these nanostructures were calculated based on the wavelength change of the incident light in the visible and near infrared region. Moreover, the height change, waveleng...
متن کاملFabrication and Characterization of the Fiber Optical Taper for a Surface Plasmon Resonance Sensor
For a fiber optical surface plasmon resonance (SPR) sensor a short part of its cladding should be removed to coat a thin layer of a metal. Usually this is problematic when an optical fiber with small core diameter is used. In this paper, a new method using µliter droplet of the HF acid for short fiber optical taper fabrication is reported. Using this method in a multi-mode optical fiber w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Analytical chemistry
دوره شماره
صفحات -
تاریخ انتشار 2016